Evidence library

Evidence & research

Every claim in the library is tied to the literature. Here are all 82 research summaries in one place, each rated for evidence strength and linked to the underlying studies. Search by supplement or filter by how strong the evidence is.

How we rate evidence
Strong

22

Moderate

47

Preliminary

12

Anecdotal

1

Evidence

Showing 82 of 82 studies

Tongkat AliModerate

Tongkat Ali and testosterone

Reviews of human trials report increases in testosterone, especially in men with low baseline levels.

Systematic review · 2022View study
Vitamin D3Moderate

Vitamin D and respiratory infections

Supplementation appears to modestly lower the risk of acute respiratory infections, with the largest benefit in deficient individuals.

BMJ meta-analysis · 2021View study
MagnesiumModerate

Magnesium and sleep

Reviews suggest modest improvements in sleep parameters, especially in older adults or those with low intake.

Systematic reviews · 2021View study
CiticolineModerate

Citicoline and attention

Randomized trials report improved attention and psychomotor speed in healthy adults and adolescents.

Randomized trials · 2021View study
AshwagandhaModerate

Ashwagandha and stress

Standardized extracts reduce perceived stress and cortisol versus placebo in several RCTs.

RCT meta-analyses · 2021View study
CollagenModerate

Collagen peptides and skin aging

A review of randomized trials found oral collagen improved skin hydration, elasticity, and wrinkle depth.

Systematic review · 2021View study
AshwagandhaModerate

Ashwagandha and sleep

Trials report improvements in sleep quality and onset latency.

Sleep trials · 2020View study

MTHFR variants and folate metabolism

The C677T and A1298C MTHFR polymorphisms reduce enzyme activity by 40–70%, impairing conversion of folic acid and raising homocysteine levels.

Genetic epidemiology studies · 2020View study
NACModerate

NAC and OCD / compulsive behaviours

Multiple RCTs show significant symptom reduction in OCD, trichotillomania, and other compulsive disorders with 1,200–2,400 mg/day.

RCTs · 2020View study

B12 deficiency in vegans and older adults

B12 deficiency is prevalent in vegans without supplementation and in older adults with reduced gastric acid.

Epidemiological studies · 2020View study

Vitamin D and bone fracture prevention

Pooled trials suggest vitamin D with calcium reduces fracture risk in older adults, particularly those who are deficient.

Meta-analyses of RCTs · 2019View study

Omega-3 and triglycerides

Consistent dose-dependent reductions in triglycerides across many trials.

Cardiology reviews · 2019View study

Omega-3 and depression

EPA-predominant formulations show modest benefit as an adjunct in depressive symptoms.

Psychiatry meta-analyses · 2019View study
L-TheaninePreliminary

L-theanine and stress

Small studies suggest reduced stress and improved relaxation markers.

Psychology studies · 2019View study
CalciumModerate

Calcium, vitamin D, and fractures

Combined calcium and vitamin D reduces fracture risk, especially in institutionalized older adults.

Meta-analyses · 2019View study
Folate (B9)Moderate

Homocysteine reduction with methylfolate

Active folate forms (methylfolate, folinic acid) are more effective than folic acid alone at lowering homocysteine in MTHFR carriers.

Intervention studies · 2019View study
GlycineModerate

Glycine and collagen synthesis

Glycine supplementation increases collagen synthesis markers, supporting joint and skin health.

Collagen research · 2019View study
L-ArginineModerate

Arginine and erectile dysfunction

Supplemental arginine (3–6 g/day) improves erectile function scores in mild to moderate ED in multiple controlled trials.

RCTs · 2019View study
CollagenModerate

Collagen for joint pain

Trials in athletes and osteoarthritis patients report reduced joint pain with daily collagen peptides.

Randomized trials · 2019View study
CreatinePreliminary

Creatine and cognition

Early studies suggest cognitive benefits, particularly under stress such as sleep deprivation.

Neuroscience trials · 2018View study
CaffeineStrong

Caffeine as an ergogenic aid

Consistently enhances endurance and high-intensity exercise performance.

Sports-science reviews · 2018View study
ProbioticsModerate

Probiotics and IBS

Some strains improve IBS symptoms, though benefits are strain- and individual-specific.

Gastroenterology reviews · 2018View study
Folate (B9)Moderate

L-Methylfolate and depression

L-Methylfolate as an adjunct to antidepressants improves outcomes in patients with major depressive disorder, particularly those with MTHFR polymorphisms.

RCTs and open-label trials · 2018View study
NACStrong

NAC and glutathione

Oral NAC reliably increases blood and tissue glutathione levels across a wide range of populations and conditions.

Clinical trials · 2018View study

B12 and homocysteine reduction

B12 supplementation lowers homocysteine, especially when combined with folate and B6.

Cochrane review · 2018View study
GlucosamineModerate

Glucosamine sulfate for knee osteoarthritis

Reviews find glucosamine sulfate offers small benefits for pain and function, with the sulfate form outperforming hydrochloride.

Systematic reviews · 2018View study

Psyllium and cholesterol

Meta-analyses confirm psyllium lowers LDL and total cholesterol in a dose-dependent way.

Meta-analysis · 2018View study
Rhodiola RoseaPreliminary

Rhodiola and stress

A standardized extract improved self-reported stress and related symptoms over several weeks in stressed adults.

Open-label and controlled studies · 2018View study

Ginseng and fatigue

Reviews of randomized trials report reductions in mental and physical fatigue with Panax ginseng versus placebo.

Systematic review · 2018View study
Vitamin K2Moderate

Vitamin K2 and bone density

Some trials report improved bone mineral density and reduced fracture markers, though results vary by population and dose.

RCTs and reviews · 2017View study
ZincModerate

Zinc and the common cold

Lozenges started within 24 hours of symptoms may shorten cold duration, though dose and form matter.

Cochrane-style reviews · 2017View study
IronModerate

Alternate-day iron dosing

Spacing doses may increase fractional absorption by reducing hepcidin spikes.

Absorption studies · 2017View study
CreatineStrong

Creatine for strength and power

Decades of trials confirm improvements in strength, power, and lean mass with resistance training.

Sports-science reviews · 2017View study
CiticolineModerate

Citicoline and age-related cognition

Reviews suggest benefits for memory and cognition in older adults and in post-stroke recovery.

Systematic review · 2017View study
ProbioticsModerate

Probiotics and antibiotic-associated diarrhea

Specific strains reduce the risk of antibiotic-associated diarrhea in reviews.

Cochrane reviews · 2017View study
NACModerate

NAC and COPD

High-dose NAC reduces exacerbations and improves lung function in chronic obstructive pulmonary disease.

Meta-analyses · 2017View study
L-GlutamineModerate

Glutamine and gut permeability

Supplementation reduces markers of intestinal permeability in critically ill patients, surgical patients, and endurance athletes.

Clinical and exercise trials · 2017View study
L-CitrullineModerate

Citrulline malate and resistance exercise

8 g citrulline malate before training significantly increases repetitions to failure and reduces muscle soreness 24–48 h post-exercise.

RCTs · 2017View study

Riboflavin for migraine prevention

High-dose riboflavin (400 mg/day) reduces migraine frequency in adults and children.

RCTs and reviews · 2017View study

B6, homocysteine, and cardiovascular risk

B6 supplementation lowers homocysteine, especially when combined with folate and B12.

Cochrane review · 2017View study
InulinModerate

Inulin and gut bacteria

Trials show inulin-type fructans increase Bifidobacteria and support a healthier microbiome.

Systematic review · 2017View study

Rhodiola and fatigue

Standardized extracts reduced symptoms of stress-related and chronic fatigue versus placebo in multiple randomized trials.

Randomized trials · 2017View study
MagnesiumModerate

Magnesium and metabolic health

Higher dietary magnesium is consistently associated with improved insulin sensitivity and lower type-2 diabetes risk.

Cohort studies & meta-analyses · 2016View study
IronStrong

Iron and fatigue in deficiency

Supplementation improves fatigue and work capacity in iron-deficient individuals, even without full anemia.

Clinical trials · 2016View study
CaffeineStrong

Caffeine and cognitive performance

Improves alertness, vigilance, and reaction time, especially when fatigued.

Cognitive studies · 2016View study
CurcuminModerate

Curcumin and osteoarthritis

Trials suggest reduced joint pain and improved function, often comparable to NSAIDs with fewer GI effects.

Rheumatology trials · 2016View study
L-TryptophanModerate

Tryptophan and sleep

Supplemental tryptophan modestly shortens sleep latency and increases sleep efficiency in several small controlled trials.

Sleep research · 2016View study
L-ArginineModerate

Arginine and blood pressure

Meta-analysis of RCTs shows arginine supplementation significantly reduces systolic and diastolic blood pressure.

Meta-analysis · 2016View study
Vitamin K2Preliminary

Vitamin K2 and arterial calcification

Preliminary trials suggest MK-7 may slow arterial stiffening, but larger outcome studies are needed.

Vascular health trials · 2015View study
ZincStrong

Zinc and copper balance

Sustained high-dose zinc induces copper deficiency, supporting combined supplementation.

Nutrition reviews · 2015View study
CopperModerate

Copper-zinc balance

Maintaining copper intake offsets the copper depletion seen with sustained high-dose zinc.

Nutrition reviews · 2015View study

Folate and neural tube defect prevention

Periconceptional folate supplementation reduces neural tube defect risk by 50–70% in clinical trials and population studies.

Cochrane review · 2015View study
L-TyrosineModerate

Tyrosine and stress-induced cognitive decline

Supplementation attenuates the decline in working memory and information processing seen under cold-stress and sleep deprivation.

Military and cognitive stress trials · 2015View study
L-GlutamineModerate

Glutamine and immune function

Perioperative glutamine supplementation reduces infection rates and hospital stay duration in multiple clinical trials.

Surgical trials · 2015View study

Citrulline vs arginine for blood arginine levels

Oral citrulline raises plasma arginine levels more effectively than equimolar arginine due to better intestinal absorption and reduced splanchnic extraction.

Pharmacokinetic studies · 2015View study
BoronModerate

Boron and mineral metabolism

Boron supplementation reduces urinary calcium and magnesium loss and supports vitamin D status.

Controlled studies · 2015View study
BoronPreliminary

Boron and hormones

Short human trials report increases in free testosterone and changes in estrogen and inflammatory markers with daily boron.

Human trials · 2015View study
Vitamin CStrong

Vitamin C and iron absorption

Co-ingestion markedly increases non-heme iron absorption in controlled studies.

Nutrition trials · 2014View study
L-TryptophanModerate

Tryptophan, serotonin, and mood

Acute tryptophan depletion reliably worsens mood in vulnerable individuals, supporting the role of the serotonin pathway.

Depletion studies · 2014View study

Niacin and lipid profile

High-dose niacin favourably alters HDL, triglycerides, and LDL in dyslipidaemia.

Cardiovascular trials · 2014View study

Pantethine and lipids

Pantethine supplementation shows modest reductions in LDL and triglycerides in some trials.

Intervention studies · 2014View study

Psyllium for constipation and IBS

Soluble fiber from psyllium improves stool consistency and symptoms in constipation and IBS.

Systematic review · 2014View study
Vitamin CModerate

Vitamin C and the common cold

Regular supplementation modestly shortens cold duration but does not reliably prevent colds.

Cochrane review · 2013View study
Huperzine APreliminary

Huperzine A and cognition

Reviews of trials in Alzheimer's disease and cognitive decline report memory benefits, but caution that study quality is low.

Systematic review · 2013View study
PiperineModerate

Piperine as a bioavailability enhancer

Increases absorption of several nutrients and drugs by inhibiting their metabolism and transport.

Pharmacology reviews · 2013View study
L-TyrosineModerate

Tyrosine and working memory

Improves working memory and cognitive flexibility in demanding but not routine cognitive tasks.

Controlled trials · 2013View study

Betaine and athletic performance

Several RCTs report gains in power, strength, or body composition with ~2.5 g/day betaine, though findings are inconsistent across protocols.

Resistance-training trials · 2013View study

Thiamine deficiency and neuropathy

Thiamine deficiency is a well-documented cause of peripheral neuropathy and Wernicke encephalopathy.

Neurology reviews · 2013View study
Tongkat AliModerate

Tongkat Ali and stress hormones

A standardized extract reduced cortisol and improved mood scores in moderately stressed adults.

Randomized trial · 2013View study
GlycineModerate

Glycine and sleep quality

3 g of glycine before bed improves subjective sleep quality and reduces daytime sleepiness and fatigue in controlled trials.

Controlled trials · 2012View study
UridineAnecdotal

Uridine for mood

Small early studies, sometimes combined with omega-3s, suggest possible mood benefits but are limited in size and quality.

Pilot studies · 2011View study
L-TheanineModerate

L-theanine, caffeine, and attention

The combination improves attention and reduces task-switching errors more than caffeine alone.

Cognitive trials · 2010View study
5-HTPModerate

5-HTP and serotonin synthesis

5-HTP readily crosses the blood-brain barrier and is decarboxylated to serotonin, raising central serotonin more directly than tryptophan.

Mechanistic reviews · 2010View study

Ginseng and cognition

Trials suggest Panax ginseng can improve some aspects of cognitive performance and mood, especially under stress or fatigue.

Randomized trials · 2010View study
UridinePreliminary

Uridine, DHA, and synaptic membranes

Mechanistic and animal studies show uridine combined with DHA and choline increases synaptic membrane phospholipids and synaptic proteins.

Mechanistic and animal studies · 2008View study
GlucosaminePreliminary

Glucosamine and joint space narrowing

Long-term trials suggest glucosamine sulfate may slow joint space narrowing in the knee.

Long-term RCTs · 2008View study
InulinPreliminary

Inulin and mineral absorption

Prebiotic fibers can improve calcium absorption and bone mineral density in some studies.

Controlled trials · 2007View study
Huperzine AModerate

Huperzine A mechanism

Pharmacology studies confirm it is a reversible inhibitor of acetylcholinesterase that crosses the blood-brain barrier.

Pharmacology studies · 2006View study

Betaine and homocysteine

Betaine supplementation lowers plasma homocysteine in a dose-dependent manner via BHMT-mediated remethylation, independent of folate status.

Controlled trials / reviews · 2005View study
5-HTPPreliminary

5-HTP and mood

Small controlled trials suggest 5-HTP may improve low mood, but evidence quality and trial sizes are limited.

Clinical trials · 2002View study
CurcuminStrong

Curcumin bioavailability with piperine

Piperine substantially increases curcumin absorption in pharmacokinetic studies.

Pharmacology studies · 1998View study

Biotin for brittle nails

2.5 mg/day biotin improved nail thickness and reduced splitting in people with brittle nails.

Controlled trials · 1993View study

Reviewed by Vital Matrix Research Team. Research summaries are plain-language interpretations of the cited sources and are for educational purposes only, not medical advice. Many links point to PubMed searches so you can review the wider body of evidence yourself.