VitaminsStrong

Vitamin B2 (Riboflavin)

Also known as Riboflavin, Vitamin G

Riboflavin is the precursor to FAD and FMN, two coenzymes that drive mitochondrial energy production, antioxidant recycling, and the metabolism of other B vitamins. It is one of the most evidence-backed supplements for migraine prevention at high doses.

By the Vital Matrix Editorial TeamReviewed by the Vital Matrix Research TeamLast updated June 25, 2026

Benefits

Reduces migraine frequency

Strong

400 mg/day of riboflavin has been shown in trials to reduce migraine frequency and duration.

Supports mitochondrial energy

Strong

FAD and FMN are essential electron carriers in the mitochondria.

Recycles glutathione and activates other B vitamins

Moderate

Riboflavin is needed to regenerate glutathione and to convert B6 and folate into their active forms.

Pros & cons

Pros

  • Strong evidence for migraine prevention
  • Critical for activating B6 and folate
  • Very safe and inexpensive

Cons

  • High-dose migraine protocol requires 400 mg, which can be hard to find
  • Bright urine can be alarming until you know it is harmless

Side effects

Bright yellow urine

High-dose riboflavin turns urine a harmless neon yellow.

Mild

GI upset at high doses

Doses above 200 mg may cause nausea or diarrhoea in some people.

Mild

How to take it

Typical dose

1–1.5 mg RDA; 200–400 mg for migraine prevention

Timing

Morning or divided with meals; high doses with food

Tip: Take consistently for 2–3 months to judge migraine benefit.

Forms: which one to choose

The main forms of Vitamin B2 (Riboflavin) compared by absorption, tolerability, and best use.

What the research says

Riboflavin for migraine prevention

Strong

High-dose riboflavin (400 mg/day) reduces migraine frequency in adults and children.

RCTs and reviews · 2017View research

How it connects

Relationships between Vitamin B2 (Riboflavin) and other supplements in the matrix.

Synergy
Magnesium

Riboflavin and magnesium are a commonly recommended migraine-prevention pair.

Compare the pairing
Cofactor
Folate (B9)

Riboflavin is required to convert folate into its active 5-MTHF form.

Compare the pairing
See it on the matrix

Frequently asked questions

What is Vitamin B2 (Riboflavin) used for?

Vitamin B2 (Riboflavin) is most commonly used for energy, brain health, and antioxidant. 400 mg/day of riboflavin has been shown in trials to reduce migraine frequency and duration.

How much Vitamin B2 (Riboflavin) should I take?

A typical dose is 1–1.5 mg RDA; 200–400 mg for migraine prevention. Take consistently for 2–3 months to judge migraine benefit. Start at the lower end and confirm the right dose for you with a healthcare professional.

When is the best time to take Vitamin B2 (Riboflavin)?

Recommended timing: Morning or divided with meals; high doses with food.

What are the side effects of Vitamin B2 (Riboflavin)?

Reported side effects include bright yellow urine and gi upset at high doses. High-dose riboflavin turns urine a harmless neon yellow.

More Vitamins

Compare Vitamin B2 (Riboflavin) with

Weighing your options? See how Vitamin B2 (Riboflavin) stacks up against similar supplements.