Cofactor

Folate (B9) and Vitamin B2 (Riboflavin)

Riboflavin is required to convert folate into its active 5-MTHF form.

By the Vital Matrix Editorial TeamReviewed by the Vital Matrix Research TeamLast updated June 25, 2026

Designed to work as a pair

One is required for the other to do its job, so they belong together in a stack.

How to take them

Folate (B9)

Dose
400–1,000 mcg DFE per day; up to 1,000 mcg L-Methylfolate for MTHFR
Timing
With or without food; morning is common to support daytime methylation

Vitamin B2 (Riboflavin)

Dose
1–1.5 mg RDA; 200–400 mg for migraine prevention
Timing
Morning or divided with meals; high doses with food

Frequently asked

Can you take Folate (B9) and Vitamin B2 (Riboflavin) together?

Yes, they are commonly taken together. Riboflavin is required to convert folate into its active 5-MTHF form.

What's the best time to take Folate (B9) and Vitamin B2 (Riboflavin)?

Around the same time works well. Folate (B9): With or without food; morning is common to support daytime methylation. Vitamin B2 (Riboflavin): Morning or divided with meals; high doses with food.

How do Folate (B9) and Vitamin B2 (Riboflavin) interact?

Cofactor: Required for the other to do its job. Riboflavin is required to convert folate into its active 5-MTHF form.

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